ATP-binding properties of P glycoprotein from multidrug-resistant KB cells.
نویسندگان
چکیده
The photoaffinity reagent 8-azido-alpha-[32P]ATP was used to label a protein of 170 kDa in membrane vesicle preparations from a highly multidrug-resistant cell line, KB-V1, but not from the drug-sensitive parental cell line KB-3-1. The 170-kDa labeled protein was immunoprecipitated with a monoclonal antibody (MRK-16) to P glycoprotein. Both ATP and GTP inhibited labeling by 8-azido-alpha-[32P]ATP. Labeling of P170 was not inhibited by 5 mM ADP, 5 mM ribose-5-phosphate, or 100 microM vinblastine. These data directly demonstrate that P glycoprotein has a nucleotide-binding site that could supply energy for drug transport.
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ورودعنوان ژورنال:
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology
دوره 1 1 شماره
صفحات -
تاریخ انتشار 1987